Sreekanth Chalasani

Sreekanth Chalasani, PhD

Assistant Adjunct Professor
Division of Biological Sciences
Section of Neurobiology

Contact Information

Email: schalasani@salk.edu
Phone: 858.453.4100 x2117

Mailing Address:
Molecular Neurobiology Lab
The Salk Institute for Biological Studies
10010 N. Torrey Pines Rd.
La Jolla, CA 92037

Lab Website

Research Title
Dissecting neural circuits regulating behavior in C.elegans and Zebrafish.

Research Description
“Our brains respond to changes in our environment with an almost infinite repertoire of behaviors. I am interested in understanding how neural circuits sense and process information to generate behaviors.”

Our brain contains roughly 100 billion cells, each connected through thousands of contact points adding up to at least a quarter of a million miles of wiring—enough to reach from here to the moon. This marvel of evolutionary engineering allows us to navigate an ever-changing environment, to learn, and to remember, but its stunning complexity makes it difficult to trace how information travels from one neuron to another. We use the nematode Caenorhabditis elegans as a model to understand how neural circuits transform sensory input into behaviors. C. elegans has exactly 959 adult somatic cells and 302 neurons connected by approximately 6,000 electrical and chemical connections. Despite its simplicity, this animal displays a number of sophisticated behaviors, making it the perfect model to explore how a simple, well-defined nervous system is able to integrate information from multiple sensory neurons and remember it for long periods of time.

Wild-type worms spend about 15 minutes searching for food when they are moved from a plate with food to a food-free plate. The duration of this search time is a function of the quality of the food and the amount of time they have spent feeding on it before being moved (more nutritious food or longer times elicit longer searches and vice versa). We have found that the worms’ main olfactory neurons, known as AWC neurons, are primarily responsible for this food-seeking behavior. They are activated upon removal of a stimulus and respond with the release of chemical signals, which in turn, activate one target neuron (AIB) and inhibit a second target neuron (AIY). These opposite connections resemble the first connections in the visual processing system in our own eyes—photoreceptors connecting to “ON” and “OFF” bipolar cells.

In the future, we plan to extend our studies to zebrafish larvae to test whether vertebrate and invertebrate circuits use similar mechanisms to process information, hoping to gain new insight into how the human brain functions.

Chalasani, S.H., Kato, S., Albrecht, D.R., Nakagawa, T., Abbot, L.F., and Bargmann, C.I. Neuropeptide feedback modifies odor-evoked dynamics in C.elegans olfactory neurons.Nature Neuroscience 13,615-621 (2010).

Tian, L., Hires, S.A., Mao, T., Huber, D., Chiappe, M.E., Chalasani, S.H., Petreanu, L., Akerboom, J., Schreiter, E.R., Bargmann, C.I., Jayaraman, V., Svoboda, K., and Looger, L.L. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nature Methods 6, 875-881 (2009).

Macosko, E.Z., Pokala, N., Feinberg, E.H., Chalasani, S.H., Butcher, R.A., Clardy, J., and Bargmann, C.I. A hub-and-spoke circuit drives pheromone attraction and social behaviour in C.elegans. Nature 458, 1171-1175 (2009).

Tsunozaki, M., Chalasani, S.H., and Bargmann, C.I. A behavioral switch: cGMP and PKC signaling in olfactory neurons reverses odor preference in C. elegans. Neuron 59, 959-971 (2008).

Chalasani, S.H., Chronis, N., Tsunozaki, M., Gray, J.M., Ramot, D., Goodman, M.B., and Bargmann, C.I. Dissecting a neural circuit for olfactory behaviour in Caenorhabditis elegans. Nature 450, 63-70 (2007).

Chalasani, S.H.*, Sabol, A.*, Xu, H., Gyda, M.A., Rasband, K., Granato, M., Chien, C.-B., and Raper, J.A. Stromal cell-derived factor (SDF-1) antagonizes Slit/Robo signaling in vivo. * Equal contribution. J. Neuroscience 27, 973-980 (2007)

Kreibich, T.A.*, Chalasani, S.H.*, and Raper, J.A. The neurotransmitter glutamate reduces the axonal responsiveness to multiple repellents through the activation of mGluR1. * Equal contribution. J. Neuroscience 24, 7085-7095 (2004).

Chalasani, S.H., Baribaud, F., Coughlan, C.M., Sunshine, M.J., Lee, V.M.Y., Doms, R.W., Littman, D.R., and Raper, J.A. The chemokine Stromal cell-Derived Factor-1 promotes the survival of embryonic retinal ganglion cells. J. Neuroscience 23, 4601-4612 (2003).

Chalasani, S.H., Sabelko, K.A., Sunshine, M.J., Littman, D.R., and Raper, J.A. The chemokine, SDF-1, reduces the effectiveness of multiple axonal repellents and is required for normal pathfinding. J. Neuroscience 23, 1360-1371 (2003).

Vatamanuik, O.K., Mari, S., Lang, A., Chalasani, S., Demkiv, L.O., and Rea, P.A. Phytochelatin synthase, a dipeptidyl transferase that undergoes multisite acylation with y-glutamylcysteine during catalysis. Stoichiometric and site-directed mutagenic analysis of AtPCS1-catalyzed phytochelatin synthesis. J. Biol. Chem 279, 22449-22460 (2004).