People

Theodore Friedmann, A.B., M.D.

Theodore Friedmann, A.B., M.D.

Kathryn Bouic, B.A.

Kathryn Bouic, B.A.

Hanna Palmer

Hanna Palmer

Ghiabe Guibinga, Ph.D.

Ghiabe Guibinga, Ph.D.

Shaochun Song, M.D., Ph.D.

Shaochun Song,
M.D., Ph.D.

Ling Guo, M.D., Ph.D.

Ling Guo, M.D., Ph.D.

Stephen Hsu, B.S.

Stephen Hsu, B.S.

Selena Gillis

Selena Gillis

Eric Maxwell

Eric Maxwell

Al Pollard

Al Pollard

 

The Friedmann laboratory has long been interested in the development of the concepts and techniques of human gene therapy. These interests have taken the form of work in a variety of gene transfer vector systems, most notably the development of the VSV-G pseudotyping methods of retroviruses and more recently the development of self-assembled nanoparticles that enhance gene transfer of naked plasmids or liposomes through the incorporation of envelope protein receptor ligands such as VSV-G or other viral envelope glycoproteins. Previous disease models of interest have included neoplastic, cardiovascular and CNS disorders and now focus on Lesch Nyhan Disease, a neuro-developmental disorder caused by deficiency of the purine biosynthetic enzyme hypoxanthine guanine phosphoribosyltransferase (HPRT) and associated with disordered dopamine neurotransmission in the brain. We are approaching this complex monogenic disease by extensive characterization of the effects of HPRT gene mutations on global gene expression patterns and on the proteome of affected tissues. To understand how the brain development of the dopamine neurotransmitter system goes awry in HPRT deficiency, we are now applying these molecular methods to a study of normal and HPRT-deficient human embryonic stem cells as they differentiate into dopaminergic neurons.

We are also currently carrying out similar extensive studies of the effects of growth factors and anabolic steroids on gene expression and on the proteomes of muscle cells in culture and on mouse muscle and other tissues in vivo. These studies are aimed partly at developing molecular screening methods for gene-based doping in sport.

 

Dr. Friedmann

Theodore Friedmann, A.B., M.D.

University of Pennsylvania and M.A., University of Oxford.

Contact: tfriedmann@ucsd.edu

Kathy Bouic

Kathryn Bouic, B.A.

University of California, San Diego

B.A. Microbiology

Laboratory manager.

Contact: kbouic@ucsd.edu, (858)534-5245

Hannah Palmer

Hanna Palmer

Administrative Assistant. Mrs. Palmer holds everything together.

Raman Bhasker, Ph.D.

Vikram University, India. Research positions at Indian Institute of Science, Bangalore; University of Texas Southwestern Medical Center; Adelaide Universitty Commonwealth Advanced Center for Gene Technology; Flinders University, Adelaide; and Case Western University, Cleveland.

Dr. Bhasker is leading the studies on transcriptional effects of growth factor (IGF-1) action.

Ghiabe Guibinga

Ghiabe Guibinga, Ph.D.

Dr. Guibinga is directing much of the laboratory's efforts in the development of virosomes and other non-viral nanoparticles for gene transfer.

Shaochun Song

Shaochun Song, M.D., Ph.D.

Dr. Song is directing the studies on the transcriptional effects of HPRT and gene expression studies of human embryonic stem cells in their differentiation pathway to dopaminergic neurons.

Ling Guo

Ling Guo, M.D., Ph.D.

University of Copenhagen

Dr. Guo is currently working on projects related to understanding the effects of aging on neurodegeneration on brain energy and purine metabolism and is currently characterizing mitochondrial function in HPRT deficiency.

Atsushi Miyanohara, Ph.D.

Dr. Miyanohara directs the vector production laboratory of the UCSD Program in Gene Therapy.

Charles C. King, Ph.D.

Dr. King is leading the effort in the proteomic characterization of HPRT-deficient tissues and also the proteomic effects of IGF-1 and other growth factors in vitro and in vivo. He is also studying the differentiation of human embryonic stem cells and human fetal islet cells to glucose-responsive insulin-producing pancreatic islet cells.

Steven Hsu

Stephen Hsu, B.S.

University of California, San Diego

Lab assistant. Steven is working with Dr. Guibinga in cell culturing studies dealing with HPRT. He is a graduate student in UCSD's Integrated BS/MS Program working on a Master's Degree in Biology.

Selena Gillis

Selena Gillis

University of California, San Diego

Lab Assistant, Webmaster

 

Contact: sgillis@ucsd.edu

 

Eric Maxwell

Eric Maxwell

University of California, San Diego

Lab Assistant. Eric is working with Dr. Charles C. King and Dr. Marygorret Obonyo on characterization of H. Pylori interaction with PDK1 & Akt cell signaling pathways. He is currently a Biochemistry/Chemistry undergraduate with specialization in protein chemistry.

All Pollard

Al Pollard

Custodian